The PEX 1 ATPase stabilizes PEX 6 and plays essential roles in peroxisome

Short title: 1 Plant PEX1 is essential for peroxisomes 2 3 Corresponding author: 4 Bonnie Bartel, Rice University, MS-140, 6100 Main Street, Houston, Texas 77005; Phone: 7135 348-5602; E-mail: [email protected] 6 7 8 9 The PEX1 ATPase stabilizes PEX6 and plays essential roles in peroxisome biology 1 10 11 Mauro A. Rinaldi 2 , Wendell A. Fleming, Kim L. Gonzalez, Jaeseok Park 3 , Meredith J. Ventura 4 , 12 Ashish B. Patel 5 , and Bonnie Bartel* 13 14 Department of BioSciences, Rice University, Houston, Texas 77005. 15 16 One sentence summary: 17 Novel missense alleles of the Arabidopsis PEX1 ATPase reveal essential peroxisomal roles that 18 impact embryogenesis and plant growth. 19 20 Author contributions: 21 M.A.R., W.A.F., K.L.G., and B.B. conceived and planned the experiments. M.A.R. performed 22 most of the experiments and wrote the first draft of the manuscript. W.A.F. organized the screen 23 that isolated pex1-2, analyzed pex1-2 genomic sequencing data, and obtained pex1-2 plants 24 expressing GFP-PTS1. K.L.G. constructed plasmids for overexpressing PEX1 and PEX6. J.P. 25 mapped pex1-3 and assisted with mutant characterization. M.J.V. isolated pex1-2 and conducted 26 pex1-2 recombination mapping. A.B.P. assisted with mutant characterization. All authors revised 27 the manuscript and approved the final version. 28 29 Plant Physiology Preview. Published on June 9, 2017, as DOI:10.1104/pp.17.00548